DNA sequence (Open Reading Frame - ORF)


or upload a FASTA file

Select starts/stops



Design primers

(with )



5'-FW overhang-3'
filler
restriction site
start codon
5'-RV overhang-3'
filler
restriction site
stop codon

5'-FW overhang-3'

5'-RV overhang-3'

Overhangs

Annealing


Primers

        Tip: You can copy-paste primers in spreadsheets.

Polypeptides

    
MW pI ε